1143 Hydrogenated retinol attenuates the UVB-stimulated expression of neprilysin by interrupting epithelial-mesenchymal cytokine interactions

Over-expression of the fibroblast elastase neprilysin (NPL) plays a pivotal role in impairing three-dimensional architecture dermal elastic fibers, which results loss skin elasticity and subsequent wrinkle formation (WF). The expression level NPL human fibroblasts (HDFs) is closely associated with epithelial-mesenchymal cytokine interactions (EMCI) via IL-1α GM-CSF, both have potential to stimulate by HDFs. Since we recently found that topical application hydrogenated retinol (HR) significantly improved wrinkling symptoms facial clinical study, characterized mode action HR association mechanism. When HDFs were co-cultured UVB-exposed primary keratinocytes (HPKs), abrogated increased mRNA levels Analyses cytokines revealed while co-culture stimulated secretion IL-1α, GM-CSF IL-6, those three cytokines. While but not or IL-6 HPKs, conditioned medium at activity protein levels, was attenuated post-UVB treatment HR. there no direct inhibitory effect on activity, these suggest could interrupt EMCI IL-1a abrogate On other hand, IL-1α-treated HDFs, abolished GM-CSF. Taken together, indicate abrogating attributable its UVB-increased HPKs GM-CSF-stimulated Our be an effective agent ameliorate WF down-regulation EMCI.